Purification and Crystallization of Hepatic Glucokinase and Glucokinase Regulatory Protein
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منابع مشابه
Glucokinase and glucokinase regulatory protein: mutual dependence for nuclear localization.
Conditional expression of the glucokinase regulatory protein in insulinoma cells, under control of the reverse tetracycline-dependent transactivator, was used to investigate whether expression of this protein de novo would alter the intracellular distribution of glucokinase. The regulatory protein, which was undetectable in the basal state, could be induced by doxycycline to levels comparable t...
متن کاملIntracellular distribution of hepatic glucokinase and glucokinase regulatory protein during the fasted to refed transition in rats.
We have studied the intracellular distribution in vivo of glucokinase (GK) and glucokinase regulatory protein (GKRP) in livers of fasted and refed rats, using specific antibodies against both proteins and laser confocal fluorescence microscopy. GK was found predominantly in the nucleus of hepatocytes from starved rats. GK was translocated to the cytoplasm in livers of 1- and 2-h refed animals, ...
متن کاملAffinity Chromatography Applied to the Purification of Rat Hepatic Glucokinase
Anderson, S. R. & Weber, G. (1965) Biochemistry 4, 1948-1957 Brodelius, P. & Mosbach, K. (1973) FEBS Lett. 36, 223-226 From, H. J. (1963) J. Biol. Chem. 238,2938-2944 Lowe, C. R., Harvey, M. J. &Dean, P. D. G. (1974) Eur. J . Biochem. 41,347-351 Mosbach, K., Guilford, H., Ohlsson, R. & Scott, M. (1972) Biochem. J. 127,625-631 OCarra, P. & Barry, S. (1972) FEBS Lett. 21,281-285 Ohlsson, R., Brod...
متن کاملEvolution of glucose utilization: glucokinase and glucokinase regulator protein.
Glucose is an essential nutrient that must be distributed throughout the body to provide energy to sustain physiological functions. Glucose is delivered to distant tissues via be blood stream, and complex systems have evolved to maintain the levels of glucose within a narrow physiological range. Phosphorylation of glucose, by glucokinase, is an essential component of glucose homeostasis, both f...
متن کاملRole of Glucokinase in the Subcellular Localization of Glucokinase Regulatory Protein
Glucokinase (GCK) is the rate-limiting enzyme of liver glucose metabolism. Through protein-protein interactions, glucokinase regulatory protein (GCKR) post-transcriptionally regulates GCK function in the liver, and causes its nuclear localization. However the role of GCK in regulating GCKR localization is unknown. In the present study, using in vitro and in vivo models, we examined the levels o...
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ژورنال
عنوان ژورنال: The FASEB Journal
سال: 2006
ISSN: 0892-6638,1530-6860
DOI: 10.1096/fasebj.20.5.a960-b